Service overview

Rapid identification of bacteria, yeasts, and filamentous fungi by MALDI-TOF MS (protein “fingerprint” profiling) using the Biotyper platform. Results are reported at species level when confidence thresholds are met; otherwise, genus level or “no reliable identification” is reported.

Sample requirements (critical)

• Pure, fresh cultures in exponential phase grown on an appropriate agar medium.

• Provide for each isolate: internal code, microorganism type (bacterium/yeast/mould), source of isolation, date, culture medium, and culture conditions (temperature, respiration, incubation time), plus any remarks.

• Indicate whether fresh cultures can be supplied.

• For pathogenic bacteria, a single-use target plate is mandatory.

Workflow & deliverables

1. Spotting (with on-target formic-acid extraction when required), matrix addition, MALDI-TOF acquisition.

2. Database matching and quality review.

3. Deliverables: PDF report with organism name, match score/confidence, and notes on any limitations or retests.

Turnaround & pricing notes

• Routine isolates are typically processed promptly after receipt; difficult organisms may require extraction or repeat runs.

• 2,500–4,000 DZD per isolate depending on preparation complexity (e.g., extraction) and repeat measurements.

Limitations

• Mixed/old/low-biomass cultures and improper media can yield low scores or failed IDs; resubmission may be requested.

• Highly unusual or database-poor taxa may only resolve to genus.

How to order

Submit the EGTP-IMT request form with all requester details and isolate metadata; sign the ethical responsibility declaration included in the form.

Genomic DNA extraction is a critical procedure used to isolate DNA from cells or tissues for downstream applications such as PCR, sequencing, and cloning. The process typically involves several steps: cell lysis, removal of contaminants, DNA precipitation, DNA purification, and quantification/quality check.

Service overview

Accurate taxonomic identification of bacteria, archaea, and fungi (and, upon request, plants/animals) via PCR amplicon Sanger sequencing using universal markers (e.g., 16S rRNA, ITS, 18S rRNA). Workflow includes DNA extraction, PCR amplification, amplicon cleanup, and sequencing (F or F+R). Results are interpreted against reference databases to report species-level ID when quality thresholds are met.

Sample requirements (critical)

• Pure, recent culture or suitable biomass/tissue for DNA extraction.

• For each sample provide: internal code, organism type (bacterium/yeast/mould/other), source of isolation, date, culture medium, and culture conditions (temperature, respiration, incubation time), plus any relevant remarks.

• Avoid mixed cultures and aged/overgrown plates; these can reduce amplification and read quality.

Workflow & deliverables

1. DNA extraction → PCR with universal primers (region per organism type).

2. Amplicon cleanup → Sanger sequencing (F only or F + R).

3. Quality review and database comparison.

4. Deliverables: ABI chromatograms, FASTA sequences, and a brief sequencing QC summary (coverage/quality; ID confidence notes).

Turnaround & pricing notes

• Turnaround: up to 15 working days from sample receipt (depends on workload/complexity).

• Pricing: 6,700 DZD per sample (Forward only). 10,000–12,000 DZD per sample (Forward + Reverse) reflecting amplicon length/GC content, cleanup complexity, and any necessary re-runs.

Limitations

• Mixed/contaminated or low-quality DNA may yield ambiguous reads or failed amplification.

• Some taxa/regions may not resolve to species; in such cases, genus-level ID or “no reliable identification” may be reported.

How to order

Submit the EGTP-Seq02 request with sample metadata and desired target region (if known). Indicate whether you prefer Forward only or Forward + Reverse sequencing.

Service: Custom DNA primer synthesis on a MerMade 4 phosphoramidite solid-phase oligosynthesizer.

Specs

• Length: 18–40 nt (customer-specified).

• Purification: Butanol desalting (removes small-molecule reagents; suitable for routine PCR/Sanger).

• Delivery concentration: 1 µM in nuclease-free water (default; dried pellets available on request).

• QC: OD260 yield and sequence/length report; advanced QC (e.g., MS) available as add-ons.

What you get

• One primer set = forward + reverse primer, synthesized and butanol-desalted, delivered at 1 µM.

• Basic design check (Tm/GC%, hairpins/dimers) upon request.

Price

• 5,000 DA per primer set (forward + reverse) with standard QC and butanol purification.

This service provides high-accuracy DNA sequencing using the Sanger method, performed directly on PCR products supplied by the applicant. PCR amplification is not included as part of this service. The focus is exclusively on sequencing, delivering reliable results for a wide range of applications, including gene identification, mutation detection, and validation studies.

Applicants must provide:

• Pure, high-quality amplicons

• Quality control (QC) results

• Appropriate sequencing primers

A comprehensive genetic sequencing package ideal for mutation detection, gene identification, and targeted screening. This service includes:

• Primer design and synthesis

• DNA extraction

• PCR amplification & cleanup

• High‑quality Sanger sequencing

Note: Bioinformatics analysis is not included.

Service overview

The Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA sequences, enabling their visualization, quantification, or use in downstream applications such as sequencing or cloning. The service involves targeted amplification using user-specified or validated primers on a high-precision thermal cycler to ensure specificity and reproducibility.

Sample requirements (critical)

To ensure reliable results, please provide the following for each reaction:

• ≥10 µL of template DNA (concentration 50–300 ng/µL)

• 5 µL of each primer (Forward and Reverse, 10 µM working concentration)

• Metadata for each sample:

  • Internal code

  • DNA origin and type (plasmidic, chromosomal, etc.)

  • Extraction method

  • Target gene and expected amplicon size

  • Primer sequences (5’–3’) and melting temperature (Tm)

  • Any relevant remarks (e.g., inhibitors, GC-rich template)

    PCR

Workflow & deliverables

1. Reaction setup using supplied or validated primers.

2. Amplification using an optimized PCR program on a gradient thermal cycler.

3. Verification of amplification by agarose gel electrophoresis (with size marker of choice).

4. Deliverables:

   • Gel image confirming product presence and size.

   • Optional recovery of PCR product in specified volume.

Turnaround & pricing notes

• Turnaround: up to 10 working days after sample receipt.

• Pricing: 1,500 DZD per reaction.

  • Price may vary with choice of PCR kit, quality control technique, or product recovery volume (as indicated in the request form).

Limitations

• Low-quality or contaminated DNA may result in weak or failed amplification.

• Primers with mismatches, hairpins, or high GC content may require redesign or gradient optimization.

How to order

Submit the EGTP-PCR request form with all required sample and project information.
Ensure that DNA and primers are supplied according to the stated volumes and concentrations, and sign the ethical responsibility declaration confirming compliance with applicable biosafety and ethical regulations

Nucleic Acid Quality Control

Code: EGTP-CAN | Type: Analysis | Price: 500 DZD per sample

Service overview

Assessment of purity, concentration, and integrity of nucleic acids (DNA/RNA) to ensure suitability for downstream applications (PCR, cloning, sequencing). Measurements are performed by spectrophotometry (ScanDrop) and/or agarose gel electrophoresis as requested.

Sample requirements (critical)

• DNA: ≥20 µL at 5–100 ng/µL in nuclease-free water or TE.

• RNA: ≥20 µL at 10–100 ng/µL in RNase-free water/TE; keep on ice/≤4 °C and ship promptly.

• Provide for each sample: internal code, nucleic acid type (DNA/RNA), source/organism, extraction method/kit, and any additives (e.g., EDTA, salts).

Workflow & deliverables

1. Spectrophotometry (ScanDrop):

   • Concentration (A260), A260/280 and A260/230 purity ratios.

2. Agarose gel electrophoresis (optional/if requested):

   • Integrity/fragmentation check and approximate size profile.

3. Deliverables: 1-page QC report (per sample) with measured values, pass/fail comments for common downstream uses, and gel image when applicable.

Turnaround & pricing notes

• Turnaround: typically 2–5 working days from sample receipt.

• Pricing: 500 DZD per sample (spectrophotometry only).

  • Add +500 DZD if gel integrity check is requested (optional).

  • Large batches may be scheduled based on instrument availability.

Limitations

• Spectrophotometry can overestimate concentration in the presence of contaminants (phenol/salts); if high accuracy is required (e.g., NGS), request a fluorometric assay (quoted separately).

• Degraded or contaminated samples may be flagged as not recommended for downstream applications.

How to order

Submit the EGTP-CAN request indicating DNA or RNA, whether you require gel assessment, and the intended downstream application (PCR, Sanger, WGS) so we can apply appropriate acceptance thresholds.

Microbial Whole Genome Sequencing (Illumina MiSeq)

Code: EGTP-Illumina-Microbial-WGS | Type: Analysis | Price: Starting from 67,000 DZD*

*Final pricing depends on reagent market variations and required sequencing coverage (depth), which differ per organism and genome size.

Service overview

Whole genome sequencing (WGS) provides complete genomic characterization of bacterial and fungal isolates using Illumina MiSeq high-throughput sequencing technology. This service supports microbial taxonomy, antimicrobial-resistance gene detection, comparative genomics, phylogenetics, and outbreak surveillance.

Includes:
✔ DNA extraction from pure culture
✔ DNA quantity/quality assessment
✔ Illumina library preparation
✔ Paired-end MiSeq sequencing
✔ Raw data delivery and QC metrics

Bioinformatics and downstream analysis are optional add-on services (e.g., assembly, annotation, AMR analysis).

Sample requirements (critical)

To ensure high-quality sequencing:

• Pure, fresh, isolated colonies on suitable agar (e.g., TSA, Sabouraud)

• 4 sterile tubes × 5 mL liquid medium for regrowth prior to DNA extraction

• Clearly labeled with isolate code, source, organism type

• Biosafety declaration required for clinical or pathogenic isolates

  • Include biosafety level and risk classification

  • Samples with undeclared biological risk are not accepted

Workflow & deliverables

1. Culture verification and DNA extraction

2. DNA QC (yield, purity, integrity)

3. Illumina library and sequencing run

4. Deliverables:

   • Clean FASTQ files (paired-end)

   • Summary QC report: read count, Q-scores, estimated coverage

   • Optional bioinformatics analysis (quote on request)

Turnaround & pricing notes

• Turnaround: typically 15–30 working days depending on sequencing queue and coverage requirements

• Pricing:

  • from 67,000 DZD for standard bacterial WGS (baseline coverage)

  • Higher coverage or complex genomes (e.g., fungi) = adjusted pricing

  • Price may fluctuate with Illumina reagent costs and project design

Limitations

• Mixed isolates or poor DNA quality can reduce sequencing performance

• Fungi and large genomes require deeper coverage and extended processing

How to order

Submit a WGS request form including:

• Sample identifiers & complete metadata

• Organism type and biosafety information

• Required sequencing depth / analysis goals

Ensure compliant transport and biosafety packaging according to institutional and national guidelines.