Service Overview

Rapid and reliable identification of bacteria, yeasts, and filamentous fungi using MALDI-TOF mass spectrometry (protein fingerprint profiling) on the MALDI Biotyper platform.
Microorganisms are identified by comparing their protein mass spectra to validated reference databases.

Results are reported at the species level when confidence score thresholds are met. If thresholds are not reached, identification is reported at the genus level or classified as “no reliable identification.”

Sample Requirements (Critical)

• Pure, fresh cultures in the exponential growth phase, grown on an appropriate solid agar medium (2% agar, 20 g/L).

• Well-isolated colonies are required (e.g., TSA, Sabouraud, or equivalent, depending on the microorganism).

• Cultures must be delivered within 24 hours after incubation, maintained at 4–8 °C during transport.

For each isolate, the requester must provide:

• Internal sample code

• Microorganism type (bacterium / yeast / mould)

• Source of isolation (environmental, food, clinical, etc.)

• Date of isolation

• Culture medium

• Culture conditions (temperature, respiratory type, incubation time)

• Any relevant remarks

The requester must indicate whether fresh cultures can be supplied. If unavailable, purification and subculturing may be performed by PLAGENOR, subject to additional charges.

🔴 Important: Without fresh, pure cultures, reliable identification cannot be guaranteed.

Pathogenic or Clinical Isolates

For pathogenic or clinical isolates, submission of a biological risk declaration specifying the biosafety level (e.g., BSL-2 or BSL-3) is mandatory.

• Single-use disposable MALDI target plates are compulsory for these samples.

• Additional costs apply for disposable targets and enhanced biosafety handling.

• Any undeclared biological risk will result in automatic rejection of the request.

Workflow & Deliverables

• Direct spotting of colonies on MALDI target plates

• On-target formic acid extraction when required

• Matrix application and MALDI-TOF spectral acquisition

• Automated database matching and expert quality review

Deliverables include:

• A PDF report for each isolate containing:

  • Identified organism name

  • Confidence score

  • Interpretation notes (limitations, need for retesting, or recommendations)

Turnaround & Pricing Notes

• Routine isolates are processed promptly upon receipt.

• Difficult isolates may require additional extraction steps or repeat measurements.

Indicative pricing (per isolate):

Standard (non-pathogenic) cultures

• Simple analysis: 2,500 DZD

• Duplicate: 4,500 DZD

• Triplicate: 6,500 DZD

Pathogenic / clinical isolates

• Simple analysis: 4,000 DZD

• Duplicate: 7,000 DZD

• Triplicate: 10,000 DZD

Final cost depends on sample type, analysis mode (simple/duplicate/triplicate), and any additional processing required.

Limitations

• Mixed cultures, aged colonies, low biomass, or unsuitable growth media may result in low confidence scores or failed identification.

• Rare or poorly represented taxa in reference databases may only be identified at the genus level.

• Resubmission may be recommended in such cases.

How to Order

Complete and submit the EGTP-IMT request form with all required requester information and isolate metadata, and sign the ethical responsibility declaration included in the form.

Genomic DNA extraction is a critical procedure used to isolate DNA from cells or tissues for downstream applications such as PCR, sequencing, and cloning. The process typically involves several steps: cell lysis, removal of contaminants, DNA precipitation, DNA purification, and quantification/quality check.

Service Overview

Accurate taxonomic identification is performed using PCR amplicon Sanger sequencing with universal genetic markers, including 16S rRNA for bacteria, ITS for fungi, and 18S rRNA for eukaryotes.

The workflow comprises PCR amplification, amplicon cleanup, and single-direction (Forward) or bi-directional (Forward + Reverse) Sanger sequencing. Generated sequences are compared against curated reference databases to provide species-level identification when established quality criteria are met.

Sample Requirements (Critical)

Samples must consist of either a pure, recent culture suitable for PCR (preferred) or extracted DNA meeting the following specifications: A260/280 ratio of approximately 1.8–2.0, DNA concentration of at least 10–20 ng/µL, and a minimum volume of 20 µL.

Each sample must be accompanied by complete metadata, including sample code, organism type, source, date, culture medium, incubation conditions, and any relevant remarks. Mixed cultures, contaminated samples, or degraded DNA should be avoided, as they can negatively affect amplification and sequencing quality.

Workflow and Deliverables

PCR amplification using universal primers selected according to the organism group.
Amplicon cleanup followed by Sanger sequencing (Forward only or Forward + Reverse).
Sequence quality review and database comparison.

Deliverables include ABI chromatogram file(s), FASTA sequence file(s), and a brief sequencing quality control summary describing read quality, coverage, and confidence of identification.

Pricing (Algerian Dinar – DA)

Sequencing Service with PCR Included (per sample)

• Forward sequencing (F only): 7,000 DA
  (5,500 DA sequencing + 1,500 DA PCR)

• Forward + Reverse sequencing (F + R): 12,000 DA
  (10,500 DA sequencing + 1,500 DA PCR)

• Forward + Reverse sequencing for long, high-GC, or re-run amplicons: 13,500 DA
  (12,000 DA sequencing + 1,500 DA PCR)

Additional Services (Optional)

• Fungal samples requiring lyophilisation: +2,000 DA per sample

• Primer design (depending on DNA template complexity): +2,000 to +5,000 DA

Turnaround Time and Pricing Notes

The standard turnaround time is up to 15 working days from sample receipt, depending on workload and sample complexity.

Rates are subject to change based on reagent availability and fluctuations in reagent prices. Any pricing adjustments will be communicated prior to order confirmation.

Limitations

Mixed or contaminated samples, degraded DNA, or challenging genomic regions may result in ambiguous sequencing reads or failed amplification. In some cases, certain taxa may not resolve to species level.

How to Order

Submit the EGTP-Seq01 request form with complete sample metadata and the preferred target region (if known). Please indicate whether Forward-only or Forward + Reverse sequencing is required, along with any optional add-on services.

Service Overview

Accurate taxonomic identification of bacteria, archaea, and fungi (and, upon request, plants or animals) using PCR amplicon Sanger sequencing with universal genetic markers such as 16S rRNA, ITS, and 18S rRNA.

The workflow includes DNA extraction, PCR amplification, amplicon cleanup, and single-direction (Forward) or bi-directional (Forward + Reverse) Sanger sequencing. Generated sequences are compared against curated reference databases to provide species-level identification when sequence quality and coverage criteria are met.

Sample Requirements (Critical)

Clients must provide pure, recent cultures or suitable biomass/tissue for DNA extraction.

For each sample, the following metadata is mandatory:

• Internal sample code

• Organism type (bacterium, yeast, mould, or other)

• Source of isolation

• Date of isolation

• Culture medium

• Culture conditions (temperature, respiration, incubation time)

• Any relevant remarks

Mixed cultures, contaminated samples, or aged/overgrown material should be avoided, as they significantly reduce DNA quality, amplification success, and sequencing accuracy.

Workflow and Deliverables

DNA extraction from the submitted sample.
PCR amplification using universal primers selected according to organism group.
Amplicon cleanup followed by Sanger sequencing (Forward only or Forward + Reverse).
Quality assessment and database comparison.

Deliverables include:

• ABI chromatogram file(s)

• FASTA sequence file(s)

• A brief sequencing QC summary indicating read quality, coverage, and confidence of identification

Pricing (Algerian Dinar – DA)

The following prices are based on EGTP-Seq01 rates, with PCR (1,500 DA) and DNA extraction (1,000 DA) included per sample.

Final all-inclusive prices per sample:

• Forward sequencing (F only): 8,000 DA
  (5,500 DA sequencing + 1,500 DA PCR + 1,000 DA DNA extraction)

• Forward + Reverse sequencing (F + R): 13,000 DA
  (10,500 DA sequencing + 1,500 DA PCR + 1,000 DA DNA extraction)

• Forward + Reverse sequencing for long, high-GC, or re-run amplicons: 14,500 DA
  (12,000 DA sequencing + 1,500 DA PCR + 1,000 DA DNA extraction)

Optional add-on services:

• Fungal samples requiring lyophilisation: +2,000 DA per sample

• Primer design (if required, depending on template complexity): +2,000 to +5,000 DA

Turnaround Time and Pricing Notes

Turnaround time is up to 15 working days from sample receipt, depending on workload and sample complexity.

Rates are subject to change based on reagent availability and reagent price fluctuations. Any pricing adjustments will be communicated prior to order confirmation.

Limitations

Mixed or contaminated samples, degraded DNA, or difficult genomic regions may result in ambiguous sequencing reads or failed amplification. Certain taxa or target regions may not resolve to species level; in such cases, results may be reported at the genus level or as “no reliable identification.”

How to Order

Submit the EGTP-Seq02 request form with complete sample metadata and the preferred target region (if known). Clearly indicate whether Forward only or Forward + Reverse sequencing is required, and specify any optional add-on services.

Service: Custom DNA Primer Synthesis

Overview

Custom DNA primer synthesis performed on a MerMade 4 phosphoramidite solid-phase oligonucleotide synthesizer, suitable for routine PCR and Sanger sequencing applications.

Technical Specifications

• Primer length: 18–40 nucleotides (customer-specified)

• Synthesis platform: MerMade 4 phosphoramidite solid-phase oligosynthesizer

• Purification: Butanol desalting
  (removes small-molecule synthesis reagents; appropriate for routine PCR and Sanger sequencing)

• Delivery format:

  • 1 µM in nuclease-free water (default)

  • Dried pellets available upon request

• Quality control (QC):

  • OD260 yield measurement

  • Sequence and length report

  • Advanced QC (e.g. mass spectrometry) available as optional add-ons

What You Receive

• One primer set, consisting of a forward and a reverse primer

• Primers synthesized, butanol-desalted, and delivered at 1 µM

• Basic in-silico design check (Tm, GC%, hairpins, primer-dimers) available upon request

Pricing (Algerian Dinar – DA)

The total service cost ranges from 8,500 to 14,000 DA, depending on primer length and design complexity.

Base primer synthesis (per primer set: forward + reverse):

• Primer length up to 25 nucleotides: 8,500 DA

• Primer length 26–30 nucleotides: 11,000 DA

• Primer length 30–40 nucleotides: 14,000 DA

Primer design (optional, added to synthesis price):

• Simple DNA templates (small or well-characterized genomes): +2,000 DA

• Moderate-complexity templates: +3,000–4,000 DA

• Complex templates (large genomes, eukaryotic or highly repetitive, requiring high-specificity design): +5,000 DA

Pricing Notice

• Rates are subject to change depending on reagent availability and reagent price fluctuations.

• Any price adjustment will be communicated prior to order confirmation.

Service Description

This service provides high-accuracy DNA sequencing using the Sanger method, performed directly on PCR amplicons supplied by the applicant. PCR amplification is not included in this service. The scope is limited exclusively to sequencing, ensuring reliable and reproducible results for applications such as gene identification, mutation detection, and sequence validation.

Applicant Requirements

Applicants must provide the following:

• Pure, high-quality PCR amplicons

• Quality control (QC) results for the supplied amplicons

• Appropriate sequencing primers

Pricing (Algerian Dinar – DA)

Sequencing service (per sample):

• Forward sequencing (F only): 5,500 DA

• Forward + Reverse sequencing (F + R): 10,500 DA

• Forward + Reverse sequencing for long, high-GC, or re-run amplicons: 12,000 DA

Turnaround Time and Pricing Notes

Turnaround time is up to 15 working days from sample receipt, depending on workload and sample complexity.

Rates are subject to change based on reagent availability and reagent price fluctuations. Any price adjustment will be communicated prior to order confirmation.

A comprehensive genetic sequencing package ideal for mutation detection, gene identification, and targeted screening. This service includes:

• Primer design and synthesis

• DNA extraction

• PCR amplification & cleanup

• High‑quality Sanger sequencing

Note: Bioinformatics analysis is not included.

Service overview

The Polymerase Chain Reaction (PCR) is a molecular biology technique used to amplify specific DNA sequences, enabling their visualization, quantification, or use in downstream applications such as sequencing or cloning. The service involves targeted amplification using user-specified or validated primers on a high-precision thermal cycler to ensure specificity and reproducibility.

Sample requirements (critical)

To ensure reliable results, please provide the following for each reaction:

• ≥10 µL of template DNA (concentration 50–300 ng/µL)

• 5 µL of each primer (Forward and Reverse, 10 µM working concentration)

• Metadata for each sample:

  • Internal code

  • DNA origin and type (plasmidic, chromosomal, etc.)

  • Extraction method

  • Target gene and expected amplicon size

  • Primer sequences (5’–3’) and melting temperature (Tm)

  • Any relevant remarks (e.g., inhibitors, GC-rich template)

    PCR

Workflow & deliverables

1. Reaction setup using supplied or validated primers.

2. Amplification using an optimized PCR program on a gradient thermal cycler.

3. Verification of amplification by agarose gel electrophoresis (with size marker of choice).

4. Deliverables:

   • Gel image confirming product presence and size.

   • Optional recovery of PCR product in specified volume.

Turnaround & pricing notes

• Turnaround: up to 10 working days after sample receipt.

• Pricing: 1,500 DZD per reaction.

  • Price may vary with choice of PCR kit, quality control technique, or product recovery volume (as indicated in the request form).

Limitations

• Low-quality or contaminated DNA may result in weak or failed amplification.

• Primers with mismatches, hairpins, or high GC content may require redesign or gradient optimization.

How to order

Submit the EGTP-PCR request form with all required sample and project information.
Ensure that DNA and primers are supplied according to the stated volumes and concentrations, and sign the ethical responsibility declaration confirming compliance with applicable biosafety and ethical regulations

Nucleic Acid Quality Control

Code: EGTP-CAN | Type: Analysis | Price: 500 DZD per sample

Service overview

Assessment of purity, concentration, and integrity of nucleic acids (DNA/RNA) to ensure suitability for downstream applications (PCR, cloning, sequencing). Measurements are performed by spectrophotometry (ScanDrop) and/or agarose gel electrophoresis as requested.

Sample requirements (critical)

• DNA: ≥20 µL at 5–100 ng/µL in nuclease-free water or TE.

• RNA: ≥20 µL at 10–100 ng/µL in RNase-free water/TE; keep on ice/≤4 °C and ship promptly.

• Provide for each sample: internal code, nucleic acid type (DNA/RNA), source/organism, extraction method/kit, and any additives (e.g., EDTA, salts).

Workflow & deliverables

1. Spectrophotometry (ScanDrop):

   • Concentration (A260), A260/280 and A260/230 purity ratios.

2. Agarose gel electrophoresis (optional/if requested):

   • Integrity/fragmentation check and approximate size profile.

3. Deliverables: 1-page QC report (per sample) with measured values, pass/fail comments for common downstream uses, and gel image when applicable.

Turnaround & pricing notes

• Turnaround: typically 2–5 working days from sample receipt.

• Pricing: 500 DZD per sample (spectrophotometry only).

  • Add +500 DZD if gel integrity check is requested (optional).

  • Large batches may be scheduled based on instrument availability.

Limitations

• Spectrophotometry can overestimate concentration in the presence of contaminants (phenol/salts); if high accuracy is required (e.g., NGS), request a fluorometric assay (quoted separately).

• Degraded or contaminated samples may be flagged as not recommended for downstream applications.

How to order

Submit the EGTP-CAN request indicating DNA or RNA, whether you require gel assessment, and the intended downstream application (PCR, Sanger, WGS) so we can apply appropriate acceptance thresholds.

Microbial Whole Genome Sequencing (Illumina MiSeq)

Code: EGTP-Illumina-Microbial-WGS | Type: Analysis | Price: Starting from 85,000 DZD*

*Final pricing depends on reagent market variations and required sequencing coverage (depth), which differ per organism and genome size.

Service overview

Whole genome sequencing (WGS) provides complete genomic characterization of bacterial and fungal isolates using Illumina MiSeq high-throughput sequencing technology. This service supports microbial taxonomy, antimicrobial-resistance gene detection, comparative genomics, phylogenetics, and outbreak surveillance.

Includes:
✔ DNA extraction from pure culture
✔ DNA quantity/quality assessment
✔ Illumina library preparation
✔ Paired-end MiSeq sequencing
✔ Raw data delivery and QC metrics

Bioinformatics and downstream analysis are optional add-on services (e.g., assembly, annotation, AMR analysis).

Sample requirements (critical)

To ensure high-quality sequencing:

• Pure, fresh, isolated colonies on suitable agar (e.g., TSA, Sabouraud)

• 4 sterile tubes × 5 mL liquid medium for regrowth prior to DNA extraction

• Clearly labeled with isolate code, source, organism type

• Biosafety declaration required for clinical or pathogenic isolates

  • Include biosafety level and risk classification

  • Samples with undeclared biological risk are not accepted

Workflow & deliverables

1. Culture verification and DNA extraction

2. DNA QC (yield, purity, integrity)

3. Illumina library and sequencing run

4. Deliverables:

   • Clean FASTQ files (paired-end)

   • Summary QC report: read count, Q-scores, estimated coverage

   • Optional bioinformatics analysis (quote on request)

Turnaround & pricing notes

• Turnaround: typically 15–30 working days depending on sequencing queue and coverage requirements

• Pricing:

  • from 67,000 DZD for standard bacterial WGS (baseline coverage)

  • Higher coverage or complex genomes (e.g., fungi) = adjusted pricing

  • Price may fluctuate with Illumina reagent costs and project design

Limitations

• Mixed isolates or poor DNA quality can reduce sequencing performance

• Fungi and large genomes require deeper coverage and extended processing

How to order

Submit a WGS request form including:

• Sample identifiers & complete metadata

• Organism type and biosafety information

• Required sequencing depth / analysis goals

Ensure compliant transport and biosafety packaging according to institutional and national guidelines.

Service Overview

The platform provides lyophilisation (freeze-drying) of biological samples using the Beta 2-8 LSCplus freeze-dryer (Martin Christ, Germany). This process preserves samples by removing water under controlled vacuum and low-temperature conditions, resulting in a dry and stable product suitable for storage and downstream analyses.

The service includes sample reception, pre-freezing at −80 °C, lyophilisation according to adapted protocols (primary and/or secondary drying), and delivery of the dried material.
Lyophilisation parameters (freezing conditions, vacuum level, drying duration) are adjusted according to sample type, volume, and container characteristics to ensure optimal moisture removal.

General Sample Requirements (Critical)

• Samples must be pure, clearly labeled, and provided in suitable threaded containers compatible with the Beta 2-8 LSCplus, respecting recommended filling volumes.

• For pathogenic or clinical samples, a biological risk declaration specifying the required biosafety level is mandatory. Without it, the request may be suspended or refused.

• While the platform ensures pre-freezing at −80 °C, the intrinsic quality of the samples (purity, stability, concentration, absence of contamination or degradation) remains the responsibility of the requester.

Processing Time, Volume & Pricing

• Processing time: Variable, depending on sample type, volume, and lyophilisation cycle duration.

• Standard pricing basis (per sample per 24 h):

– 40 mL sample — Primary lyophilisation: 3,000 DZD / 24 h
– 40 mL sample — Secondary lyophilisation: 4,000 DZD / 24 h

• Volumes exceeding 40 mL or requiring special containers are billed proportionally.

• The total cost is calculated based on:
– Sample volume
– Drying mode (primary and/or secondary)
– Total lyophilisation duration (in 24 h cycles)

⚠️ Extended drying times (e.g. ≥48 h) are billed proportionally.