IBTIKAR

Genomics Technology Platform of Higher School of Biological Sciences of Oran

Adresse: Cité Emir Abdelkader (EX-INESSMO) 31000 Oran, Es Senia, Algeria

E-mail: genomicsplatform.essbo@gmail.com

Téléphone: 041 24 63 59

Catégorie: MESRS

Nombre de services : 8

Nombre d'équipement : 7

Nombre de prestations : 7

Description

The genomics technology platform is designed to empower PhD students, researchers and clinicians by providing comprehensive and high-precision genomic services across a wide range of applications. We specialize in microbial genomics, including advanced techniques like MALDI-TOF, PCR-based sequencing, and whole-genome sequencing with MiSeq Illumina. Additionally, our platform supports genomic research in other areas, such as metagenomics, DNA extraction, primer synthesis for PCR amplification, genetic sequence analysis, and molecular cloning. With a focus on high accuracy and fast turnaround times, our services enable in-depth exploration of microbial behavior, gene functions, genetic variations, and much more, fostering cutting-edge research and discovery across diverse fields of genomics.

Microbial identification via MALDI-TOF	EGTP-IMT	The MALDI-TOF Biotyper is used for fast and precise identification of microorganisms based on mass spectrometry analysis. It identifies microorganisms by analyzing their protein fingerprints through a Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS).
Genomic DNA extraction	EGTP-GDE	Genomic DNA extraction is a critical procedure used to isolate DNA from cells or tissues for downstream applications such as PCR, sequencing, and cloning. The process typically involves several steps: cell lysis, removal of contaminants, DNA precipitation, DNA purification, and quantification/quality check.
Molecular Identification via Sequencing (PCR Amplification and Sequencing)	EGTP-Seq01	Obtain precise taxonomic identification of bacteria, archaea, fungi, plants, or animals through sequencing with universal primers (e.g., 16S rRNA, 18S rRNA, ITS regions). This service includes PCR amplification, PCR cleanup, and high-quality sequencing to enable accurate genus and species classification. Please note: downstream bioinformatics analysis is not included.
Microbial identification via sequencing (DNA extraction, PCR amplification, and sequencing)	EGTP-Seq02	Obtain precise taxonomic identification of bacteria, archaea, fungi, plants, or animals through sequencing with universal primers (e.g., 16S rRNA, 18S rRNA, ITS regions). This service includes DNA extraction, PCR amplification, PCR cleanup, and high-quality sequencing to enable accurate classification at the genus and species levels. Please note: downstream bioinformatics analysis is not included.
Primer Synthesis	EGTP-PS	The OligoSynthesizer is used for the synthesis of oligonucleotide primers essential in polymerase chain reaction (PCR), sequencing, and other nucleic acid-based analyses. It offers the customization of primers for specific applications.
DNA Sequencing via Sanger Method	EGTP-SeqS	This service provides high-accuracy DNA sequencing using the Sanger method, performed directly on PCR products supplied by the applicant. PCR amplification is not included as part of this service. The focus is exclusively on sequencing, delivering reliable results for a wide range of applications, including gene identification, mutation detection, and validation studies. Applicants must provide: Pure, high-quality amplicons Quality control (QC) results Appropriate sequencing primers
Targeted Genetic Sequencing and Analysis	EGTP-GS	A comprehensive genetic sequencing package ideal for mutation detection, gene identification, and targeted screening. This service includes: Primer design and synthesis DNA extraction PCR amplification & cleanup High‑quality Sanger sequencing Note: Bioinformatics analysis is not included.
PCR (Polymerase Chain Reaction)	EGTP-PCR	PCR (Polymerase Chain Reaction) is a powerful technique used to amplify specific DNA sequences, enabling their analysis and identification. This service involves using specific primers to target a gene or region of interest, followed by amplification using a Thermal cycler.

MALDI-TOF MS Biotyper	PLAG19	The MALDI-TOF MS Biotyper utilizes Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry for the identification and analysis of microorganisms (bacteria, fungi, yeasts). The system works by ionizing the microbial proteins with a laser and measuring the mass-to-charge ratios of the ions produced. The resulting spectra are compared to a pre-built database containing the spectral profiles of a wide range of microorganisms. This technology allows for the identification of pathogens with high accuracy and speed, often within minutes, which is essential for clinical diagnostics and microbiology research.
Nucleic Acid Automated Extractor	PLAG24	Automated nucleic acid extractors streamline the process of isolating DNA or RNA from samples. They are highly accurate and efficient for clinical and research labs where high throughput is essential.
Thermal Cycler	PLAG35	A thermal cycler (PCR machine) automates the amplification of DNA through cycles of denaturation, annealing, and elongation. Precise temperature control is essential for optimal PCR performance, with standard systems capable of handling high-throughput multiplexed reactions, ideal for genetic analysis, diagnostics, and research.
Gel DOC System	PLAG14	A Gel Documentation System (Gel Doc) is used for imaging and analyzing agarose or polyacrylamide gels after electrophoresis. It is used in molecular biology to capture high-quality images of stained DNA or proteins in gels.
Oligo Synthesizer	PLAG25	Oligonucleotide synthesizers automate the chemical synthesis of short DNA and RNA sequences. Utilizing solid-phase synthesis with phosphoramidite chemistry, these machines enable the precise construction of oligonucleotides, commonly used in PCR primers, probes for diagnostics, and RNA interference experiments.
ScanDrop Spectrophotometer	PLAG32	A ScanDrop spectrophotometer is used for precise and rapid quantification of nucleic acids, proteins, and other biomolecules in microvolume samples without the need for cuvettes.
Sanger Sequencer (Genetic Analyzer)	PLAG31	The Sanger sequencing method is based on chain-termination of DNA synthesis. This technology uses labeled dideoxynucleotides to incorporate into a growing DNA strand, terminating the sequence at specific points. Sequencing is performed in capillary electrophoresis systems, providing high accuracy for sequencing short to medium-length DNA fragments.